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While the overall "metabolism" classifications are similar for FB and ripening clusters (21.5% vs 20.9%) the MIPS category 01.06 for lipid, fatty-acid and isoprenoid metabolism, which include the known flavour components such as terpenes, shifts from 2.6% in FB to 4.3% in the ripening cluster (data not shown).
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Similarly, the well represented classes in AZ tissue at the late stage of ripening (Cluster B) included ZF proteins, homeobox domain proteins, bHLH proteins, and bZIP proteins.
Furthermore, a vacuolar pyrophosphatase (1614834_at) putatively involved in malate vacuolar uptake was expressed early and then induced during ripening (cluster 5), as previously observed [ 73].
In particular, there were more genes downregulated throughout ripening (cluster 2) and specifically between BV to EV (cluster 4) in CT berries, whereas there were more genes downregulated specifically between EV and H (cluster 3) in C berries.
Four sugar transporters were found modulated: the hexose transporter VvHT6 (1615257_andand 1619691_at) was positively modulated during ripening (cluster 3), as previously described [ 10]; interestingly, a transcript homologous to a sugar transporter isolated in Citrus fruit was also induced while the forth transcript, which is homologous to a sugar transporter from tomato root is repressed.
Many genes coding for Aux-IAA proteins were down-regulated during ripening (VVTU17953_s_at, cluster 5, VVTU1813_at, cluster 6, VVTU7286_at, cluster 2, VVTU23500_at, cluster 5, VVTU2445_s_at, cluster 5) which may suggest that auxin levels are indeed lowered after véraison.
Genes coding for JAZ1 and JAZ8 were up-regulated during ripening (VVTU38616_s_at, cluster 8; VVTU39811_s_at, cluster 1) whereas for JAZ3 (VVTU4273_s_at, cluster 6) was down-regulated.
Interestingly, two genes involved in pre-mRNA splicing, an important mechanism of regulation of gene expression, were up-regulated during ripening (VVTU11603_at, cluster 8, VVTU28953_s_at, cluster 8).
In order to investigate the role of trace endogenous ethylene in the beginning of ripening, experimental clusters on the vine in field were selected at the 7th WAFB and divided into three groups for following treatments.
In order to investigate the role of trace endogenous ethylene in the beginning of ripening, experimental clusters on the grape plants were selected at the 7th WAFB (one week before véraison) and divided into three groups for following treatments.
In the course of ripening, the cluster thinning did not affect the berry growth.
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