Sentence examples for rinsing buffer from inspiring English sources

18 Briefly, neuron cultures were washed with phosphate-buffered saline (PBS -based rinsing buffer and incubated for 30 minutes at 4°C with 1mg/ml Sulfo-NHS-Biotin (Thermo Fisher Scientific, Waltham, MA) in rinsing buffer.

Importantly, no diazonium salt was detected in a rinsing buffer solution with pH 8; hence, the weakly alkaline media inhibits the DS desorption.

The precipitate of DAMP4var-pexiganan protein was recovered by centrifugation (38,000×g, 35 °C, 20 min), and then washed with rinsing buffer (25 mM Tris HCl, 1 M NaCl, pH 8) containing Na2SO4 at a concentration that retained the protein as a precipitate.

Thereafter, cells were rinsed once in 1× rinsing buffer provided in the kit.

Cells were rinsed three times then permeabilized in PBS containing 0.1 M glycine (rinsing buffer) plus 0.1% Triton X-100 for 5 min and again rinsed 3× in rinsing buffer.

The endogenous optineurin exhibited a diffuse cytoplasmic distribution pattern when 0.1 M glycine was included in the PBS rinsing buffer (Fig. 2A, top panels).

Organs were fixed transiently with 10% neutral-buffered formalin for 30 minutes and rinsed with rinse buffer (2 mM MgCl2, 0.1% sodium deoxycholate, 0.2% NP-40, 1× PBS, pH7.3) for 30 minutes.

Cells were collected after centrifugation and then sonicated in rinse buffer (20 mM tris HCl, 1 % Tritoon-X100, 1 mM DTT, 50 mM NaCl and 1 mM EDTA, pH 8.5) for 30 min at 300 W (1/1 s).

The middle phase was transferred into a new tube followed by addition of 5 ml rinse buffer.

The digested tumor specimens were filtered through a sterile metal grid (mesh size 440 µm) with rinse buffer, collected and centrifuged (300×g, 10 min, RT).

The supernatant was removed and cells were resuspended in 0.5 ml EGTA buffer [NaCl (8.3 g/L), KCl (0.5 g/L), 25 mM Hepes, EGTA (0.38 g/L)] and mixed with 5 ml rinse buffer.