Although this is not the first study to use duplex PCR to identify and distinguish C. dubliniensis and C. albicans, but it is the first to analyses oral samples directly through rinse pellet analysis, and samples from a Sri Lankan diabetic population using duplex PCR.
The volunteers were asked to chew the pellet for 2 min. Two minute chews were followed by the collection of the saliva and rinse water for analysis of loss of silicon particles during chewing and recording the observations.
Hybridizations, rinsing, scanning, and gene analysis on the Agilent's all human cDNA microarray (Hu44K) were conducted according to the manufacturer's protocol (G2940BA; Agilent Technologies, Inc., Palo Alto, CA).
To avoid contamination of samples, all PTFE material (Teflon vessels, pipettes, micro pipettes, tips and autosampler cups) were immersed in freshly prepared 15% v/v HNO3 for 2 h before analysis, then rinsed thoroughly with deionized water and dried in a dust free area before use.
Adherent cells were fixed in cold 4% paraformaldehyde for 15 min, rinsed and stored before analysis.
Patch roots for transcriptome analysis were rinsed and immediately frozen in liquid nitrogen.
To determine the cellulose content, the residual pellet obtained after the monosaccharide analysis was rinsed twice with ten volumes of water and hydrolysed with H2SO4 as described [ 51].
After three more TBS-T rinses, scanning and image analysis were completed with Li-Cor Odyssey Infrared Imager and Odyssey Software v.10 (Li-Cor).
In order to determine the effect of rinsing on surfactant residues, PCA analysis was performed on polystyrene cleaned with the anionic surfactant and rinsed one to five times with deionised water.
The fact of the strong sorption of piracetam in silica nanocarriers was confirmed using direct injection of the brain tissue with the nanoparticles into the dosing loop and subsequent multiple rinsing by solvent (methanol) and analysis by LC-MS (as mentioned below).
