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The most influent factors were sample amount, rinsing time, extractant volume and acid silica gel amount.
The statistically significant variables were sample amount, rinsing time, extractant volume, and acid silica amount.
If the rinsing time was set at the highest level, the boiling time hardly affected on the recovery of SCCPs.
In addition, a rinsing step in deionized water is necessary between both polyelectrolytes baths, and a drying step is performed between each rinsing time [20, 23, 24].
The rinsing time was chosen to minimize cross-adaptation between stimuli.
Then tip back and rinse another time.
Be sure to use fresh mouth rinse each time.[16].[16]
Rinse 3 times with PBS/10% FBS.
Lather and rinse three times.
Repeat this rinse 2-3 times.
Rinse several times to be thorough.
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