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Once the optimal probe concentration and hybridization conditions were optimized to reduce the background signal using untargeted probes (Figure S6 A and B), we performed FISH and stained with TIAR or DCP1, after rinsing abundantly with phosphate buffered saline to remove excess formamide.
The lipid bilayers are then rinsed abundantly with deionized water to form continuous SLBs.
The post-etch residue was removed by immersing in NMP at 80°C for 30 min and abundantly rinsing in IPA and DI water.
To improve adhesion, the initial GaN template (as received) was cleaned with NMP at 90° by sweeping the surface several times with a soft stick, then abundantly rinsing with IPA and DIW.
Briefly, immediately after obtaining each specimen of the placental tissue was denuded from decidua fragments and abundantly rinsed with 0.9 % saline to remove all blood.
After each step, the samples were filtered and the solid fraction was abundantly rinsed until reaching neutral pH, then oven dried at 60°C for 24 hours.
Entire plantlets were harvested, abundantly rinsed in 4 successive baths of ultra-pure water, quickly blotted and snap-frozen in liquid nitrogen.
Rinse well.
Rinse them.
That's becoming abundantly clear.
Rinse thoroughly".
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