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Prior to experimentation, parasite cultures were synchronized to early ring stage using 5% sorbitol, the procedure preformed twice consecutively ensuring removal of all parasites not at the ring stage.
Time-course experiments indicated that the ring stage is more rapidly inhibited, compared to the other stages.
Highly synchronous ring stage parasite was used in each assay.
After 48 h of incubation, the numbers of ring stage parasites per 5000 RBCs were determined and percentage ring stage parasitemia was calculated to assess the parasite growth.
Synchronized parasite cultures at of early ring stage parasites (6 8 h.p.i).
This method resulted in synchronisation of parasites at the ring stage, similar to previously described.
Similar(22)
RBCs containing post-ring stage parasites were classified into two classes: young trophozoites (T1) and mature trophozoites/early schizonts (T2).
Serial sectioning was also carried out on ring-stage material, using a Reichert Ultracut E ultramicrotome.
Sorbitol synchronizations were used to obtain ring-stage cultures.
B. Solubility analysis of RON12 in ring-stage parasites.
Many of the ring-stage parasites exposed to 40 °C developed further, whereas ∼75% of ring-stage parasites exposed to 41 °C did not.
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