As substrate is being translocated through the Rpt ring, the Rpn11 subunit of the 19S RP, which is positioned immediately above the channel through the Rpt ring, scans for ubiquitin chains.
For our measurements the ONH scan protocol was used which consists of 12 radial scans of 3.4 mm in length (452 A-scans each) and 6 concentric ring scans ranging from 2.5 to 4.0 mm in diameter (587 to 775 A-scans each), all centered on the optic disc.
When out walking on the South Downs, the wind in my hair, nothing but rolling hills for miles, I become Viggo Mortensen as Aragorn in The Lord of the Rings, scanning the terrain during those Monty Python-esque scenes of the fellowship running across the mountaintops of Middle Earth.
Increasing the sensitivity of the PET scanner by using 3-D acquisition (instead of 2-D acqusingion using lead or tungsten septa between crystal rings), increasing scan duration, enlarging bed overlap and raising the administered FDG activity (below the amount that results in count rates exceeding the maximum count rate capabilities of the scanner) all improve SNR.
After air-drying the collected increment cores, they were mounted on wooden holders, sanded with progressively finer grit sandpaper to highlight annual rings, and scanned with a high-resolution flatbed scanner (Microtek ScanMaker 1000XL plus at 1200 dpi).
RNA/DNA binding regulatory factors that contain many different combinations of domains, including zinc, ring, PhD, SET, Scan, and many others, were abundant in the serum/plasma.
To increase accuracy, small marks were drawn on the basal creases of the index and ring fingers before scanning.
To measure ring diameters, we scanned fluorescence intensities and determined the distances between intensity maxima (Fig. 2A,B; Table 1).
