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We first explored the possibility that the posterior nucleus might influence the direction of ring movement mobilized by blebbistatin.
1) The initial value for contractility was a 2.5-fold increase, which is reflecting the change in contractility during the whole process of ring movement and cell elongation.
Why did the authors decide to use the lower 1.5-fold change in contractility (during ring movement only) in the revised version of the manuscript?
[Editors' note: the author responses to the re-review follow.] Reviewer #1: 1) The initial value for contractility was a 2.5-fold increase, which is reflecting the change in contractility during the whole process of ring movement and cell elongation.
Live imaging of the rotation process using specific markers of each ring domain confirmed that each ring movement depends on the presence of apoptosis at its boundary, suggesting that each ring requires apoptosis to dissociate from the neighboring tissue.
As expected, the improvement (Rwp) came at the expense of some chemical sense (e.g., slight distortion in planarity of the benzene ring, movement of H atoms to nonsensical positions), but otherwise, the geometry of the independent molecules was well-preserved, confirming the correctness of the rigid body refined crystal structure.
Similar(54)
The ring movements that we observe are very robust, with surprisingly little fluctuation, given the highly dynamic nature of the ring.
However, a shuttle ring's movement is limited between a pair of binding sites.
There's a "blob" on the top right and top left of the ring, suggesting movement - "something organic", he says.
Once the proton moves away from the c-ring, its movement becomes irrelevant to the c-ring rotation.
A mobile phone, obligingly ringing between movements of the Britten, invited the charming rebuke from Gerhardt, "Perfect timing".
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com