We also present in the Methods section an experimental method to enrich long cDNA products and to rigorously combine sequencing data for these samples with non-enriched sequencing data (in the subsection headed 'Reverse transcription with sequencing primers (first adapter)').
The idea is to "graduate" people out of extreme poverty through a rigorously tested sequencing of events.
To maximize the accuracy of bacterial identification, we rigorously screened sequences to remove poor quality reads.
It is to be emphasized that accuracy of our method is strongly dependent on experimental determination of position specific information and will improve when rigorously optimized sequences are available for a protease, including information about those that are disallowed in some positions between closely related proteases [25].
We use a Bayesian method [ 33] to rigorously quantify sequence differences between homologous protein kinases in apicomplexans and other eukaryotes, and reveal contrastingly conserved features that were not apparent previously.
Also, since for the present study we merged sequences obtained with different PCR approaches (see the Materials section) and did not rigorously clone all PCR products that could not be sequenced directly, we cannot address the abundance of Sebacinales in more details here.
Here we rigorously test the phylogenetic signal of the genome signature, which consists of the genome-specific relative frequencies of dinucleotides, on 334 sequenced prokaryotic genome sequences.
Using specialized algorithms and data structures [ 24], we rigorously computed what sequences are present in both the sequence of interest and present in the background as well as the number of base changes necessary to "convert" every n-mer contained within the sequence of interest to any n-mer present in the background collection.
In "The Invention of Minus One," which considers subtraction in many forms (rewinding, repetition, erasure), three dancers (all present or former members of the Cunningham dance company) perform rigorously structured movement sequences, sharing space with a three-dimensional animated décor based on motion capture.
In order to rigorously calculate the sequence variations without bias, phylogenetic relationships among the species is usually required.
We do agree that they cannot be rigorously proven by sequence analyses since they do not come with E-values, but in that sense they arguably have more value for bench biologists to guide experiments, because they do require more expert insight and knowledge.
