Sentence examples for rigorous washing from inspiring English sources

Therefore, rigorous washing methods, such as Renografin purification, can cause serious loss of spore surface associated proteins and are not suggested for proteomic analysis [26], [27].

However, because it is likely that some vegetative cell proteins will bind accidentally to the spore surface, the rigorous washing method, as described above, was still employed.

Given that rigorous washing steps were not able to separate the microbiota from the biopsies, we hypothesize that majority of the identified bacteria are associated tightly with the stomach mucosa.

EA1 has been well known as a major S-layer component of B. anthracis vegetative cells, and it also persistently exists in the spore preparations and bind tightly to the spore surfaces even after rigorous washing.

Although EA1 could be partially washed out during the rigorous washing step in our study, the other proteins detected by SDS-PAGE apparentlyently decreased and were present as debris in the supernatant of centrifuged purified samples (Fig. 8).

After rigorous washing with buffer A, bound Endo-A was eluted using a linear gradient of 0 to 1 M NaCl in buffer A. Fractions containing the protein were pooled, concentrated and loaded on Superdex G200 size exclusion chromatography column (Amersham) equilibrated with 20 mM Tris-HCl, pH 8.0, and 200 mM NaCl.

Cleaning up to 12 brushes at a time, it starts off with a rigorous wash cycle before going into two rinses.

Some recent studies have revealed that EA1 is also retained in the proteomic profiling of rigorous washed spores and even salt/detergent washed exosporium [26], [27].

A more rigorous wash step may be able to further reduce this nonspecifically bound gold conjugate.

Unbound GTP was removed by rigorous washes, GTPases on beads were resuspended in scintillation fluid, and counts per minutes were recorded in a scintillation counter over a GST only background.

The Royal College of GPs says we can do much to prevent the spread: improved hygiene (rigorous hand washing, cleaning surfaces, loos - anything that has been in contact with an infected person) and keeping infected people away from others for at least 48 hours after symptoms end are your best bet. 3 Eating breakfast: Attempts to reverse festive weight gain often involve meal-skipping.