Rigorous assay characterization also required a standard control template.
While we could replicate these results, we detected very little fusion with the more rigorous assay of lumenal compartment mixing.
Moving to a more resistant substrate makes for a more rigorous assay but even cotton contains a little amorphous cellulose.
However, blood sample volumes on filter paper are inevitably small, and therefore, rigorous assay validation must be performed to achieve optimum sensitivity and specificity.
Therefore, oxidative DNA damage could be higher in the WBCs of workers highly exposed to asbestos fibers but more rigorous assay methodology will be required for validation.
Although 9-cis-retinoic acid does serve as a pharmacological ligand for RXR and RAR, it has not been detected in vivo with rigorous analytical assays [18].
It is therefore important to perform rigorous confirmatory assays.
Intriguingly, without Smg6, ESCs cannot differentiate as was verified by the rigorous differentiation assays in vitro and in vivo.
We reported that WRN depletion by shRNA increased cellular sensitivity to Cr VI) by a cell viability assay [11], and confirmed this result here using the more rigorous clonogenic assay.
Thus the phenotype, although easy to discern without specialized equipment, required a rigorous phenotyping assay to enable the initial identification.
