Traditionally, genomic DNA detection is relay on a rigorous DNA amplification process, which always accompanied with complicated gel electrophoresis or expensive fluorescence detection methods.
In the meantime, our approach does not need to fulfill the rigorous conditions of the amplification functions.
Towards quantitative metagenomics of wild viruses and other ultra-low concentration DNA samples: a rigorous assessment and optimization of the linker amplification method.
Duhaime, M. B., Deng, L., Poulos, B. T. & Sullivan, M. B. Towards quantitative metagenomics of wild viruses and other ultra-low concentration DNA samples: a rigorous assessment and optimization of the linker amplification method.
One should realize that a more accurate assessment on EG's lower tendency to cause nonspecific amplification will likely require more rigorous testing methods such as cloning and sequencing of a large number of different clones.
Rigorous quality measures are applied to separate PCR amplification and/or sequence errors from true polymorphisms.
This manuscript describes a rigorous evaluation of purification methods involved in RNA amplification and labelling.
To obtain this level of sensitivity we customized the design of the MIP probes and optimized a rigorous four-step protocol: sample preparation, asMIP hybridization, circularization, and amplification.
Until the advent of the Xpert MTB/RIF test, nucleic acid amplification tests (NAAT) required a specialized laboratory with rigorous quality control which can be difficult to maintain in low resource settings.
Several papers have reported that, despite the use of rigorous protocols [ 10, 88], modern human DNA contamination is prevalent in amplification products from DNA extracts of ancient specimens [ 13, 88- 91].
These observations underscore the need to identify determinants of breast cancer sensitivity to inhibitors of PDK-1 through the rigorous analysis of multiple markers, which would include PTEN mutation status, HER2 gene amplification, PI3KCA mutational status, and levels of P-Akt and phosphorylated S6K (or gene amplification tests for the relevant genes).
