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In the absence of a neighbouring hit, a genomic region of 0.1 Mb flanking the single hit was aligned with the respective ABC transporter nucleotide sequence using the more rigorous alignment tool "matcher" from the EMBOSS package.
In those cases where a neighbouring hit was absent, a flanking 0.1 Mb genomic region was aligned with the respective ABC transporter nucleotide sequence using the more rigorous alignment tool "matcher" from the EMBOSS package.
This disagreement highlights the need for a generally accepted, rigorous alignment score.
16 Our approach employs a rigorous alignment procedure (MAFFT) and tree generation method (NJ method of BS construction from MEGA 5.0) to obtain the trees.
This inconsistency suggests, for example, that the expected input polarization state based on our choice of PCCs is not the actual polarization state of the implemented system, despite rigorous alignment.
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PE reads can provide rigorous read alignment and enhance the accuracy and coverage of SNP calling and consensus sequence inference.
We have further proven this point by mapping orthologous gene pairs regulated by bidirectional promoters in twelve species using rigorous genomic alignment information [ 9].
Rigorous genomic alignment was then performed in sequential fashion against hg19, starting with SOAP2 (Li et al., 2009 ) (parameters -M 4 -v 2 -r 1).
To date, the only detailed characterization of SIDER sequences is a rigorous manual alignment of 1013 L. major (Lm) SIDER2s set forth by Bringaud et al. [ 21], while the SIDER1 subgroup has yet to be properly portrayed.
PSI-Search is similar to PSI-BLAST, but substitutes a rigorous Smith Waterman local alignment (Smith and Waterman, 1981) search strategy (SSEARCH, Pearson, 1991) to produce optimal local alignment scores from the profile PSSM.
Briefly, the method employed in the SUPFAM+ database first relates Pfam families (with or without known structures) to SCOP superfamilies using a rigorous profile-profile alignment method, AlignHUSH [ 29].
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