The only effect observed was that the anisotropic B-factors were all in the same direction for most loops in the WT (suggesting a rigid-body motion at the fast time scale) but had very different distributions in the mutant.
It is interesting to note that of A B loop residues, L31 and E32 are rigid at pH 6.5 on the micro- to millisecond time scale, but at pH 7.3 L31 and E32 are mobile on the micro- to millisecond time scale, as L31 is with Modelfree analysis at the same pH.
This result indicates that these regions show appreciable flexibility on a fast (ps to ns) time scale, whereas the rest of the HscB backbone remains quite rigid.
The study of their causes involves in different fields of physics, from astronomy for the rigid Earth nutation, through internal geophysics for the length-of-day variation at the decadal time scale, to external geophysics for seasonal polar motion.
This indicates that the NH3+ group is chemically stable over a time scale of 40 μs at room temperature and that the film is rigid enough to see its correlation with other moieties.
NMR relaxation rates (N R1, R2) and H−N heteronuclear NOE values indicated that HscB is rigid along its entire backbone except for three short regions which exhibit flexibility on a fast time scale.
For the first time, it has been shown that bioaffinity pairing with participation of immobilized on carefully designed rigid supports is very fast and the whole process of affinity separation can be realized within second time scale.
The explicit appearance of time in the formulation introduces a short but finite time scale, which permits the consideration of contact of any combination of mechanical bodies, including rigid and discretized deformable bodies.
Time-resolved fluorescence in a nanosecond time scale recorded at ϖem=24,096 cm−1 showed a monoexponential decay of 1.5 ns, which is compatible with rigid forms of stilbene derivatives.
(See the geologic time scale).
