The results show how ESCRT-I uses a combination of a rigid stalk and flexible tethers to interact with lipids, cargo, and other ESCRT complexes over a span of ∼25 nm.
Therefore, the haltere's bouncing back along the actuation direction is either due to the elasticity of the cuticles or the muscle attached to the base and it is reasonable to model the underlying actuation mechanism with a rigid stalk connected to a torsional spring at the base.
The fact that construct 3 can be expressed as a functional protein in contrast to construct 1 suggests that the rigid Tetrabrachion stalk domain is able to confer a higher level of stability compared to the GCN4-pLI domain used for constructs 1 and 2. This view is supported by the fact that under natural conditions Tetrabrachion is stable at 130°C[22], [23].
A rigid peripheral stalk would hold the 3 hexamer in a stationary position relative to the membrane.
Written in Hindi by Makrand Deshpande, and entitled "The Musk Maiden," it was translated into English and tells the story of a rigid female writer who is spiritually stalked by the dark, Dionysian aspects of her fiction.
These results suggest that the replacement of the NA stalk by more stable and rigid domains that are known to form parallel homotetrameric structures could promote greater NA stability.
The single known species, Tubipora musica, occurs on reefs in shallow waters of the Indian and Pacific oceans and is characterized by long, parallel upright polyps, or stalks, supported by a skeleton of rigid tubes of calcium carbonate.
There is a semi-rigid exoskeleton, built by tying bamboo or juncus stalks together an an internal plastic mesh, similar to polypropylene and nylon fibers.
The lance-shaped leaves, growing out from the stalk in a dense rosette, are fleshy and rigid, with gray to dark green colour.
The dominant density of the peripheral stalk in the sub-tomogram average suggests that this region is more rigid than other parts of the complex.
