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Finally, both molecular docking and a fluorescence binding assay of noscapine as a negative control reveals the same binding site on the surface of pocket-A near a rigid loop between β2 and α2 interacting with Arg164.
Here we describe optimization of two additional prion DSEs, YML of β-sheet 1 and a rigid loop (RL) linking β-sheet 2 to α-helix 2, through in silico predictions of B cell epitopes and further translation of these epitopes into PrPSc-specific vaccines.
Canonical serine protease inhibitors commonly bind to their targets through a rigid loop stabilised by an internal hydrogen bond network and disulfide bond(s).
The catalytic cysteine occurs as part of a short, more rigid loop with a more hydrophobic environment in group II cysteine desulfurases than in group I enzymes.
Despite these amino acid mismatches, including those in the "rigid loop", the CWD agents were still effective at misfolding PrPC from BHS.
However, compared to M13V, simulation of the NRVS data of the double mutant required a more rigid loop, and a slightly stronger Fe NHis bond and H-bonding network within the CXXCH loop.
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The tool consists of a curved catheter describing many rigid loops, and fixed on a plastic sheet.
Ropes with rigid loops (NF-L) may supply refuges for spat from predators and therefore, enhance the recruitment of larger individuals, although other factors like size selective settlement could play a significant role in this result.
Strikingly, S200 phosphorylation appeared to induce a similar, rigid T loop structure with S218 and S222 in a short helix.
But the cornerstone of the trial is a new form of shark net already in use in Western Australia, which features rigid plastic loops that retain their size, thereby reducing the risk of entangling fish, dolphins and turtles.
Furthermore, Ubc9 is distinct among E2s as having an extended rigid β1β2-loop.
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