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At one point the stiff-legged soldier even tilts into a circle of the rigid barrel turns of the sort used in razzle-dazzle ballet star turns.
The larger fluorescent tag eGFP is 27 kDa and has a rigid barrel shape and likely defines most of the mobility of eGFP-CaM, which exhibits only a slightly slower diffusion coefficient than eGFP.
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In particular, the tip of α310 loop reaches across the rigid β barrel making multiple contacts with PBC.
FBs were performed by passing the forceps into the bronchoscope working channel or via the rigid bronchoscope barrel, opening the forceps, advancing the bronchoscope or forceps onto the lesion, closing the forceps thus grasping, gently tugging as to tear the tissue while retrieving the forceps through the working channel or barrel, and then placing in 10% formalin.
Herein, we evaluate whether the rigid β-barrel may serve as a scaffold that can constrain the loops of a foreign protein, and thus its antigenicity.
We speculate that the increased flexibility of this α-helical porin in comparison with rigid β-barrels may be an adaptation for the heterogeneous mycolic OM, and explains its demonstrated ability to form measurable pores with phospholipid membranes.
These NOEs are totally incompatible with the well-form and rigid β-barrel structure of the SH3 domain determined by NMR in the native condition [18].
These NOEs are totally incompatible with the well-formed and rigid β-barrel structure of the SH3 domain in the native condition.
Green fluorescent protein (GFP) exhibits a rigid central β-barrel, formed by eleven β-strands with floppy loops spanning between the stands.
A 2 cm diameter rigid cylinder (20 ml syringe barrel, Plastipak; Becton Dickinson, Drogheda, Ireland) was also used.
In a rational protein design study a metal-binding site was functionally grafted on the solvent-exposed surface of the β-barrel, whereby the rigid backbone conformation permitted the spatially defined arrangement of three His side chains.
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CEO of Professional Science Editing for Scientists @ prosciediting.com