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The morphological characteristics included keratinization, basal membrane appearance, epithelial thickness, rete ridge length, adjacent rete ridge distance, and regional variation; the immunohistochemical markers included Ki67 (a proliferative marker) and Cytokeratin 19 (CK19; a stemness marker).
Triops-induced D. barbata showed an increase in microspine density at the cranial dorsal ridge (distance between 1st and 10th microspine; Table 1), a widening of the dorsal ridge, longer microspines and a sideways orientation of the 5th microspine (all P <0.001 compared to control; Table 1).
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Eight variables were measured from each stridulatory file: Length, maximal width, 1st, 2nd and 3rd quartile widths, number of ridges and inter-ridge distance in the medial and distal portions of the stridulatory file (Figure 1).
Moreover, stridulatory files of the same length can comprise different number of ridges (Figure 5.b), which directly affect the inter-ridges distance.
The results showed that the buccal mucosae from dog and pig were non-keratinized, with similar rete ridge length and distance, compared to that of humans.
Paired Student's t-tests were used to establish the differences between the medial and distal inter-ridge distances within groups.
This result is due to the smaller inter-ridge distances presented by PVE Morph 1 (Figure 6), but also to the smaller number of ridges that are rubbed during stridulatory movements (Table 2).
The file (see supplementary Figure S6) can vary in length (Figures 4 and 5.a), maximal and quartile widths (Figure 4), medial and distal inter-ridge distances (Figure 6) and number of ridges between morphs (Figure 5.b).
Nevertheless, this morph seems to rub the stridulatory file faster during stridulation production, as suggested by the tendencies for a higher pulse repetition rate and a lower mean inter-pulse interval together with larger inter-ridge distances (Figure 6, Tables 1 and 2).
Nor did "Pakistani mujahideen" capture the Indian town of Kargil during clashes in 1999, only a high mountain ridge some distance away.
The size of the gap between the costal margin and pelvic ridge (iliocostal distance) may be validated to be a surrogate measure for the presence of osteoporosis and/or vertebral fragility fractures and thereby may be a risk factor for future fracture.
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