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The individual-based spatially constrained rarefaction curve gives more idea about species richness of samples that differ in the area and sampling effort (Chiarucci et al. 2009).
Due to the different sample sizes of the two sub-groups, an allele rarefaction method was used to standardize the allelic richness of samples [56].
The fact that these taxonomic distinctness measures are statistically independent of either the sampling intensity or richness of samples [30], [32] makes them especially attractive tools for investigating structural biodiversity.
Extrapolated species richness of samples was higher at 45°C, followed by 48°C.
Regression analysis indicated that haplotypic richness of samples pooled within year increased with time (r = 0.212, P = 0.001, Fig. 1A).
The richness of samples preserved with DMSO and glycerol was significantly smaller than inoculum (T6) and T7 samples.
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Measured patterns of diversity gave partial support to the predictions of IDH, although raw species richness of sampled plots fit the predictions better than richness adjusted for differences in stem densities among treatments.
Even though species richness comparison cannot be performed at different sequencing depths, methods for interpolation and extrapolation of rarefaction curves can precisely estimate species richness diversity of samples with different number of reads, making comparison between them possible [ 29].
We applied this approach here to estimate the total richness of the samples from the target communities, which allows a direct comparison between such samples (Table 1).
Species richness of PM samples evaluated in this study was similar to individual ambient urban air samples (Franzetti et al. 2010); however, it is noteworthy that the OTUs observed in upwind PM tended to be highly variable across the 10 feed yards, whereas OTUs observed downwind of feed yards were substantially less so.
The combination of a valid epidemiological approach together with richness of biological samples should make an important contribution to the study of the system biology of breast cancer.
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Justyna Jupowicz-Kozak
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