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The plots also encompassed a gradient in functional group richness (1, 2, 3, and 4) in a factorial design.
To do this, we used a second general linear model in which we analyzed total algal biomass as a function of species richness (1 vs. 5 species), nutrient treatment (homo- vs. heterogeneous), and the two-way interaction.
A pool of 60 native plant species was used to establish a gradient of plant species richness (1, 2, 4, 8, 16, and 60) by independent random draws with replacement.
The experimental design varies the factors plant species richness (1, 2, 4, 8, and 16 species) near-orthogonal to the number of plant functional groups (1, 2, 3, and 4) and comprises 16 replicates per species-richness level except for 14 replicates at the 16-species level (because pure legume or small herb mixtures were not possible).
A pool of 60 native plant species was used to establish a gradient of plant species (1, 2, 4, 8, 16 and 60) and plant functional group richness (1, 2, 3 and 4) in a total of 82 plots of 20×20 m (Table S1; [18]).
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Species richness 2.
Brunet's index (W) quantifies lexical richness [20].
Non-native species represented more than half of the richness (27 species).
Exotic species richness m−2, cover, and community composition were strongly related to the ecosystem classification.
Understory vegetation cover, biomass, species richness (100 m−2 and 48 m−2) and Shannon's diversity index (H′) were calculated for each plot.
Furthermore, compared with Ri sites, TD had much lower diatom density, biomass, and species richness (density: 0.68 × 105 vs. 2.03 × 105 cells/L, biomass: 0.19 vs. 0.52 mg/L, species richness: 13 vs. 23).
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