Sentence examples for rich template from inspiring English sources

He describes the very rich template of Old Czech (12 slots) and examines examples from the 14th century Dalimil Chronicleto show that Old Czech had a discourse marker ti 1'indeed, really' which was distinct from the dative clitic ti 2and which occupied a unique slot in the template.

Southern blotting of long-range PCR products, optimized for amplification of a long and GC rich template, requires less DNA, as small as 15 pg [ 108], even of lower quality, and gives reliable information about repeat copy number with using a few replicate of PCRs with 180 300 pg of DNA [ 88].

Two μl of template was amplified using High Fidelity Platinum Taq (Invitrogen Cat #11304-102) and the PCRx enhancer system to facilitate amplification of CG rich template (Cat# 11495 017), with the following thermocycling conditons: 95° for 5 minutes, (95° for 30s, 58° for 30s and 72° for 45 seconds) x 45 cycles, 72° for 7 minutes, 4° indefinitely.

In addition, the enhanced thermo-stability of the Stoffel fragment results in better efficiency when used with G+C rich templates or templates having complex secondary structure [22].

In addition to ours, a number of protocols for difficult or GC rich templates are published [ 36, 37], supplemented by several commercial kits.

Several additives were tested as denaturation reagents in the PCR protocols for MIRUs, VNTR and SSRs in an attempt to improve the amplification efficiency for the GC rich templates.

Analysis of sequence data obtained from RPA-amplified libraries showed reduced bias towards GC rich templates compared to standard Illumina libraries for both P. falciparum 3D7 and the clinical isolate.

Transcription of telomeres and other G-rich sequences produces characteristic structures, called G-loops, which contain a stable RNA/DNA hybrid on the C-rich template strand, and G4 DNA interspersed with single-stranded regions on the G-rich nontemplate strand [43], [50].

Transcription of G-rich telomeric sequences results in formation of characteristic G-loop structures, which contain a stable RNA/DNA hybrid on the C-rich template strand and G4 DNA interspersed with single-stranded regions on the G-rich strand [43].

Emulsion PCR (ePCR) was performed according to standard Applied Biosystems SOLiD™ 3 System: Templated Bead Preparation Guide, with the exception that extra dATP and dTTP (Invitrogen, Carlsbad, CA, USA) were added to the aqueous phase to compensate for the AT-rich template due to bisulfite conversion.

We started by screening commercially available polymerases for tolerance to an extremely AT-rich template.