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A dashed line indicates the approximate position of the disordered glycine-rich loop of Alk.
There is also a unique positions for the DFG and glycine-rich loop positions.
Some crucial residues like Lys830 (glycine-rich loop), Val836, Ala853, Leu905 (hinge region), Cys909, Asn954, Leu956 and Ala966 were identified.
The compound induces structural change including movement of the glycine-rich loop and peptide flip between Met108-Glu109.
Thr16 from the glycine-rich loop also hydrogen bonds the 2'-phosphate, as well as the adjacent 3'-hydroxyl group.
The histidine-rich loop is replaced by a serine-rich loop for ZnT-6.
This covalent bonding pulls the glycine-rich loop toward the compound enabling additional contacts between the compound and the glycine-rich loop to form.
In ZnT6-like sequences the histidine-rich loop is substituted by a serine-rich loop and a histidine/serine-rich region is also present at the C-terminus.
This shift of the β-sheet includes the glycine-rich loop that sits above the nucleotide.
Here we directly analyzed how the flexibility of the Gly-rich loop changes during nucleotide release.
Dissociation of Mg2+ ions makes Gly-rich loop more flexible in the ADP-bound state.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com