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Functional interaction between ribosomal protein L6 and RbgA during ribosome assembly.
Many ribosomal proteins play fundamental roles in ribosome assembly [ 5].
Also, only the linezolid-dependent isolates carried mutations in the ribosomal protein L3, known to stimulate ribosome assembly.
In E. coli, this protein binds to 16S ribosomal RNA and functions in early steps of ribosome assembly [53].
Since L11 is required for ribosome assembly, knocking down this protein should result in a ribosomal stress.
GMPPNP (which terminates ribosome assembly at the 48S stage) and cycloheximide (which trap 80S ribosomal complexes) were used to confirm the 48S and 80S ribosomal peaks.
Rackham, O. et al. Hierarchical RNA processing is required for mitochondrial ribosome assembly.
Perks, K. L. et al. PTCD1 is required for 16S rRNA maturation complex stability and mitochondrial ribosome assembly.
Our work shows that LoNA is specifically localized to nucleoli and regulates rRNAs production and ribosome assembly.
Direct observation of ribosome assembly in vivo is difficult, as few intermediates have been isolated and thoroughly characterized.
Exogenously bolstering the proteostasis network increases cellular fitness in the face of challenges to ribosome assembly, demonstrating the direct contribution of orphan r-proteins to cellular phenotypes.
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