Serum leptin was assessed by RIA (human leptin RIA kit, Alta Diagnostica, Marburg, Germany).
Serum 25- OH D was determined by competitive RIA using the IDS RIA kit (Medicorp Inc).
Acth was measured by RIA using the ImmunoChem double antibody Acth I RIA kit (MP Biomedical).
For the determination of the plasma cortisol concentration a commercially available radioimmunoassay (RIA) kit (ICN Biomedicals, Carson, CA) was used.
Xin et al. [65] also used this method to determine E2 in human sera successfully, and the result showed a good correlation compared with the commercial RIA kit.
and an adiponectin RIA kit (Linco).
Serum leptin was measured using the mouse Leptin RIA Kit (#ML-82K) from Linco Research.
We measured glucose levels with a Glutest Pro (Sanwa Kagaku Kenkyusho Co., Japan), insulin and leptin by sensitive rat insulin RIA kit (SRI-13K, Millipore, Billerica, MA) and mouse leptin RIA kit (ML-82K, Millipore, Billerica, MA).
Insulin content and secretion were assessed with the Sensitive Rat Insulin RIA Kit (Linco Research) as described [68].
Insulin level was determined by radioimmunoassay (Rat insulin RIA kit, Beijing North Institute of Biological Technology, China).
A standard 125I double-antibody RIA kit (MP Biomedicals, Solon, OH, USA) was used according to the manufacturer's instructions.
