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It paves the way for the use of DsRed-based monomeric RFPs in transgenic and gene targeted approaches which often necessitate spatially and/or temporally restricted reporter expression.
Our work demonstrates the use of monomeric RFPs in mice and provides the basis for future efforts designed to incorporate RFPs into protein fusions that can be used as spectrally-distinct subcellularly-localized tags permitting high-resolution live imaging in vivo [ 12, 22].
This is an essential prerequisite to using the available suite of DsRed-derived monomeric RFPs in fusions as reporters for high-resolution in vivo imaging, and in particular, as fusion proteins exhibiting subcellular localization and acting as segmental markers of 3-dimensional space.
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In this study we created CCR2-RFP knock-in reporter mice and bred them with CX3CR1-GFP mice.
We then test the performance of CV-1 cells in propagation of a conventional recombinant Lister strain VACV, VACVL-15 RFP, in a serum-free process.
Of course the end of goal of this is a serving up a personal RFP in order make shopping data more useful, i.e. provide customers with targeted discounts based on their buying history.
Following the referendum results, the Montenegro government announced a formal public RFP in 2007 and a Montenegro-based LLC won — doMEn Ltd, a joint venture between GoDaddy.com, Afilias Limited and ME-net, Ltd.
The parasites stably expressed enhanced green fluorescence protein (EGFP) in the tachyzoite stage and red fluorescence protein (RFP) in the bradyzoite stage, under the control of the SAG1 and BAG1 promoters, respectively.
Fluorescent probes are extensively used and can be found expressed in reporter proteins (GFP, RFP), in microspheres or as dyes that are widely used for labelling cells or for monitoring gene expressions.
Remark 4.1 Using the optimality conditions of Theorem 3.2, robust fractional dual problem (RFD) for a robust fractional problem (RFP) in the convex constraint functions with uncertainty is formulated.
The board will probably not support an RFP in a time of draconian budget reductions.
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