This study compared pulmonary effects of para-aramid respirable-sized, fiber-shaped particles (RFP) (p-aramid fibrils) and chrysotile asbestos fiber exposures in rats.
RFP (P-GP inducer) and VER (P-GP antagonist) were used as positive controls; H22/ADM cells were used as blank controls.
Immunoreactive proteins were visualized using the enhanced chemiluminescence system from Pierce (Rockford, IL, U.S .. RFP (P-GP inducer) and VER (P-GP antagonist) were used as positive controls; H22/ADM cells were used as blank controls.
In brief, H22/ADM cells were incubated with RPMI1640 medium, and then RPMI1640 medium (was used as a blank control group), APS (final concentrations 0.8 mg/L, 4 mg/L, 20 mg/L, 100 mg/L, 500 mg/L), VER (P-GP antagonist, was used as positive control) 10 μmol/L, RFP (P-GP inducer, was used as positive control) 10 μmol/L for 24 h, 48 h, and 72 h before Rh-123 experiments took place.
Comparison of CX3CR1-GFP and CCR2-RFP fluorescence intensities in the various CD115+ populations from Ccr2RFP/+Cx3cr1GFP/+ mice showed that Ly6Chi cells expressed higher levels of CCR2-RFP (P = 0.016) than Ly6Clo cells and lower levels of CX3CR1-GFP (P = 0.008) (Figure 4E).
Live cell imaging of U2OS cells stably expressing G3BP-GFP (SG marker) and Dcp1-RFP (P-body marker).
To determine whether APS is involved in P-GP expression and/or its efflux function, the P-GP inducer RFP and P-GP inhibitor VER were used as positive controls, and an H22/ADM group was used as a blank control.
rLX-RFP was rescued by cotransfecting BSR T7/5 cells with the antigenomic plasmid pLX-RFP, N, P, and L support plasmids of ZJ1 strain as previously described (Liu et al. 2007).
The plasmid mec-17 p:: rfp and the entry clones containing mec-3 p and unc-54 3′utr were described by Chen and Chalfie (2015).
The osteocalcin (OC) level of NELL1-iPSC-MSCs was 3.1-fold that of RFP-iPSC-MSCs (P < 0.05).
