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Please refer to our response to "Essential Revisions" point #2 above.
As mentioned in response to "Essential Revisions" point #1, we have conducted the control experiment involving AC5 knockout tissues and report it in revised Figure 1. 2) Figure 2 reports a BRET assay designed to detect Golf activation based on free beta-gamma binding to a NanoLuc-labeled GRK2. 10 μM dopamine caused a BRET change that was reversed by 100 μM haloperidol.
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See Essential Revision Point #2.
Please see Essential Revision Point #2.
Please see Essential Revision Point #1 above.
Please see Essential Revision Point #3, where these points have been addressed.
We have tested this in an experiment (please see Essential Revision Point 3).
2) Regarding Essential Revision point #6, the authors appear to have misunderstood the concern.
[Editors' note: further revisions were requested prior to acceptance, as described below.] 1) Regarding Essential Revision point #5, this caveat should also be included in the manuscript as a discussion point.
The manuscript has been improved but there are some remaining issues that need to be addressed before acceptance, as outlined below: 1) Regarding Essential Revision point #5, this caveat should also be included in the manuscript as a discussion point.
The number of colony forming units in an exponentially growing wild-type and SeMeCo population are the same, and correspond to the total number of cells in the SeMeCo (please see Essential Revision Point 3).
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