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The rest of the analysis dealt with all 994 sequences; in particular, the data in Figure 3 (former Figure 2) includes all trunk mutations as is made explicit in the revised legend.
In this study, a national soil map of Denmark was constructed based on the FAO–Unesco Revised Legend 1990 using digital soil mapping techniques, existing soil profile observations and environmental data.
As the original taxonomic information of the SGDBE is based on the 1990 FAO-UNESCO Soil Revised Legend (FAO 1990), these names and associated information content are used in the current exercise.
Author response: Indeed, sampling without replacement was employed, and this is mentioned in the revised legend to Figure 1.
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We performed the Mann-Whitney U test and report the statistics in the revised figure legend.
Nevertheless, we now mention this possibility in the revised figure legend.
We apologize for the confusion and have improved our description of this label in the revised figure legend.
We have tested one additional D. sechellia line and one additional D. melanogaster line for the main experiments, and have clearly stated in each revised figure legend which line of D. sechellia or D. melanogaster was used in each case.
In response to Dr. Stefan Toegel's review, we have included detailed information on the number of biological and technical replicates used in the revised figure legends.
Response to Dr. Stefan Toegel In response to Dr. Stefan Toegel's review, we have included detailed information on the number of technical and biological replicates used in the revised figure legends.
The issues with the number of aligned residues, the labeling of branches etc are addressed in the revised figure legends and the alignment used for the tree construction is in Additional File 8. Regarding small GTPases, they conclude that these arose in bacteria and their eukaryotic counterparts are of mitochondrial origin.
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