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The next-generation sequencing (NGS) data obtained by re-sequencing two individual Nipponbare plants using the Illumina Genome Analyzer II/IIx and Roche 454 GS FLX were used to check sequencing errors in the revised assembly.
The CPSC advises consumers to immediately stop using the product and offered contact information for Fisher-Price so they can receive revised assembly instructions.
Submission of a revised assembly and annotations is in progress, and will be accessible under the same project ID.
Completion of the P. chabaudi AS genome sequencing and revised assembly allowed the initial cir annotation to be manually revisited.
The revised assembly, termed Os-Nipponbare-Reference-IRGSP-1.0, is now being used in updated releases of the Rice Annotation Project and the Michigan State University Rice Genome Annotation Project, thereby providing a unified set of pseudomolecules for the rice community.
A revised assembly (Sscrofa10) is being constructed from the BAC clone derived sequence together with the WGS data.
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In the revised genome assembly, 94.6% of the assembly was anchored to a chromosome.
Sequencing errors in the revised genome assembly were identified by re-sequencing the genome of two different Nipponbare individuals using the Illumina Genome Analyzer II/IIx platform.
The revised genome assembly sequence and adjusted positions of Ensembl-predicted gene locations are available in File S5, File S6, and File S7.
The revised genome assembly provides a more accurate understanding of the structure of the stickleback genome, which should aid efforts to map genes controlling stickleback phenotypes and understand genomic dynamics during stickleback evolution.
King George gave way, and new elections to the revising assembly were held.
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