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The Integrative Genomics Viewer (IGV) browser was used to independently review sequence alignments generated by the Miseq Reporter [ 21].
High-resolution structural 3D magnetization-prepared rapid gradient echo (MPRAGE) T1-weighted, turbo spin-echo (TSE) T2-weighted, and diffusion-weighted images were acquired for all infants for clinical review (sequence parameters can be found in Merchant et al. (2009)).
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Language Function: Categorization Review sequencing and demonstrate sample writing.
An adjudication committee of 3 experts, blinded to randomization arm, independently reviewed sequence data to assign linkage classification as described below.
Functional diversity is also suggested by inspection of the aldolase-like cluster in Figure 2. All of the nodes containing a Swiss-Prot reviewed sequence for 4-OH-2-ketovalerate aldolase activity are located in a single region, while the other half of the subgroup remains unexplored and may contain new functionalities.
Spectra were searched against a UniProtKB human protein database (version Oct 5 , 2010 20,259 reviewed sequences; 75,498 non-reviewed sequences) using Bioworks 3.3.1 SP1 with the SEQUEST algorithm.
Only reviewed sequences were included in the dataset.
Only referenced and reviewed sequences were included to perform further analysis.
When annotating an InterPro entry, a curator first identifies those UniProtKB/Swiss-Prot (i.e. reviewed) sequences matched by the entry that has been experimentally characterized.
The rawwere processed against the human proteome as comprised in the Swiss-Prot database (www.uniprot.org, status: Dec12th, 2012; 20.225 reviewed sequences contained) using MASCOT server version 2.3.04 (Matrix Science, Boston, MA) and Proteome Discoverer 1.4 (Thermo Fisher Scientific).
The curator has the option of reviewing sequences that may have been left out of the submission process, such that they can be edited appropriately and then later submitted.
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CEO of Professional Science Editing for Scientists @ prosciediting.com