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The nucleotide substitutions that had been identified as the principal determinants of the attenuated phenotype of the Sabin 3 strain had reverted through a U-to-C transition at nt472 in the 5'-UTR region and a C-to-U transition at nt2493 in the VP1 coding region, leading to a Thr-to-Ile amino acid substitution of residue 6 of VP1 in all 10 isolates.
We tested whether the phenotypes could be reverted through the deletion of the viral insertion using Cre recombinase (Fig. 3 B, right panels).
Intragenic suppressors of bn2 were isolated at 100- to 1000-fold lower frequency following EMS mutagenesis than typical loss of function frequency, which was interpreted by Beanan and Strome (1992) as the bn2 mutant not being reverted through simple loss of gene activity and that the gene product is likely to be essential.
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The attenuated SHIV reverted to virulence through back-mutation in the naïve, but not the vaccinated, monkeys.
However the contentious politics in South Africa had a bearing on the name and it reverted to the Osprey through the 80s and 90s - but it went on through to its last day as the Union of South Africa.
By applying a negative voltage of approximately −0.6 V, the Cu filament was dissolved or reverted to the TE through a chemical oxidation process (Cu → Cuz+ + ze–), and the device was reverted to a HRS.
Some former IDUs had reverted to drug use through coming into regular contact with current users.
Government investigators said he reverted to past behavior, trading through more than 50 nominee accounts in the name of his new wife, other relatives, even a yeshiva run by a cousin.
The developmentally committed identity of somatic cells can be reverted to a pluripotent state through different reprogramming approaches.
At later stages, the metaphase suddenly became kinked, indicating that the bipolar spindles had reverted to a tripolar spindle, likely through fragmentation of one centrosome (Supplementary Movie S10).
These mutations were reverted in the full length proteins through hydrogenolytic desulfurization of cysteine to alanine in the presence of Raney nickel.
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