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Part c in this necklace making instructions: Firstly, add 12/0 Glass Seed Bead*3 onto each wire tip and then cross two tips through one another 12/0 Glass Seed Bead; Secondly, add 12/0 Glass Seed Bead*2, 203-Topaz Swarovski Crystal Bead*1 and 12/0 Glass Seed Bead*2 onto each tip, then cross two tips through one 12/0 Glass Seed Bead again; Thirdly: reversely repeat the above two parts.
Reversely oriented items were reverse coded before data analysis.
An aliquot of 0.5 1 µg of RNA was reversely transcribed using M-MuLV Reverse Transcriptase (Finnzymes).
Total RNA was reversely transcribed using SuperScript™ III reverse transcriptase (Invitrogen GmbH, Karlsruhe, Germany).
For QPCR, total RNA was reversely transcribed using Superscript III reverse transcriptase (Invitrogen).
Items with reverse direction from the others were reversely recoded for interpretability.
RNAs were quantified and reversely transcripted into cDNA using SuperScript III Reverse Transcriptase (Invitrogen).
Immunoprecipitated mRNAs were reversely transcribed using avian myeloblastosis virus (AMV) reverse transcriptase kit (Roche Applied Sciences).
Purified RNA was reversely transcribed by murine Moloney leukemia virus reverse transcriptase using random hexamer oligonucleotides.
Total RNA (0.5 1 μg) was reversely transcribed to produce cDNA using Superscript III reverse transcriptase (Invitrogen) primed with Oligo (dT 12 18 (Invitrogen).
RNA was reversely transcribed using random hexamer priming and MMLV reverse transcriptase (Invitrogen).
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