ADH1C promoter has a single site for Oct1 and reverse sites for HNF3beta and Sp1.
The ADH3 TATA-less promoter shows single sites for Oct1, c-Myb and HNF3beta, a reverse CCAAT box and reverse sites for USF, GATA-1 and AP1.
From a total of nine sites, six sites are reversed polarity, two sites are normal polarity and pass the reversal test of McFadden and McElhinny (1990) while one site is of erratic behavior probably due to lightning strike.
DAVE KEHR Correction: October 30, 2003, Thursday A film review in Weekend on Friday about "The Party's Over," a documentary about the 2000 presidential election, reversed the sites of the two major political conventions.
Non-OLFR genes show only a slight bias of 1.2 reverse sites for every forward site per 1 kilobase.
PCR primers with EcoRI (forward) and XbaI (reverse) sites were designed to subclone TBPH RNAi construct into pMF3 vector (33).
The ADH2 promoter contains one NF1-binding site in forward orientation and two reverse sites for GATA-1, NF1 and AP1, and one for Gfi1.
For A. formosae, 11 genes were determined not to contain either forward or reverse editing sites, while for A. capillus-veneris forward and reverse editing sites were not present in 16 and 49 genes respectively.
Distance parameter between forward and reverse priming sites can be adjusted by the user (default: 400 nt).
Sequencing was performed using ABI PRISM Big Dye cycle-sequencing kit version 1.1 (Perkin Elmer, CA, USA) and the primers for the M13 forward and reverse priming sites of the pCR2.1 vector.
A total of 6 individual clones were amplified directly by PCR using M13 forward and reverse priming sites and six amplicons of the correct size were sequenced as described above using primers T3 or T7.
