Exact(2)
This search was also performed against a decoy (reversed) sequence, and ambiguous identifications were discarded.
However, further analysis of the sequence coverage of this region revealed that identical (not complementary) but reversed sequence existed in both the forward and reverse directions of the loop region of this structure (fig. 6).
Similar(58)
The reversed sequences of the target database were used as decoy database.
Both mutations were identified in the forward and reverse sequence directions.
Each forward and reverse sequence was edited using Sequencher™.
Sequence data from both forward and reverse sequence strands were used for confirmation.
All sequences were determined by the consensus of the forward and reverse sequence analysis.
Forward and reverse sequence reactions were performed with the Big Dye Terminator Cycle Sequencing Ready Reaction Kit Applied Biosystemss, Foster City, California).
Forward and reverse sequences were pasted into the inputfile.txt and primer3_core was then run on this file.
Sequences of putative lncRNAs and mRNAs were excised from the genome with samtools, and reverse sequences were reversed and complemented with the revseq –reverse –complement command.
The forward and reverse sequences were combined to obtain the full sequence of the ITS repeat.
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