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A Spectrum Mill auto-validation was first performed in the protein details, followed by peptide mode using default values [Minimum scores, minimum scored peak intensity (SPI), forward minus reversed score threshold, and rank 1 minus rank 2 score threshold].
Items with a reversed score were recoded, after which mean scores on the items of the two scales were calculated.
Four of the items are reversed score and the scale has a 5-point Likert response format.
Only spectra with a reversed score at least two fold smaller than the real score were taken into account for the auto-validation.
Similar(56)
Several of the items had reversed scoring.
Correlation is significant at p < .05 level (2-tailed) Reversed scores towards positive orientation.
Several questions were reversed scored.
Reversed scores are applied for five negatively worded items.
The reversed scored items are marked with an asterisk.
One item, asking about anxiety and depression, was reversed scored.
Items are reversed scored; higher scores always reflect better quality of life.
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CEO of Professional Science Editing for Scientists @ prosciediting.com