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Test results shows that proposed relaying schemes can provide primary as well as back-up protection to the forward and reverse line sections respectively.
HPV DNA was amplified using the consensus primers PGMY09 and PGMY11, and reverse line-blot hybridization was used to detect type-specific amplicons for 37 HPV types.
Diagnosis was confirmed by polymerase chain reaction and reverse line blot and dogs infected with Babesia canis vogeli or Ehrlichia canis were excluded.
The recently developed AdvanSure HPV GenoBlot assay (AdvanSure assay) is based on real-time PCR amplification and reverse line-blot hybridization.
The advantage of this scheme is to provide a major protection up to 99.5% of total line length using single end data and furthermore backup protection to the forward and reverse line sections.
The methods used were polymerase chain reaction (PCR) and reverse line blot (RLB) hybridization.
Thus, reverse line probe assays are used as a first step analysis tool.
Initially, we performed HPV detection and typing on all cases using nested PCR and reverse line hybridisation.
Moojen et al. (2007) published a study on the optimization and validation of a combined broad-range 16S rRNA PCR and reverse line blot hybridization protocol.
HPV testing was performed using biotinylated L1 consensus primers and reverse line blot in cervical samples from women and in genital samples from men.
For example, enzyme immunoassay, which was used in our study, is more likely to detect the excess of multiple HPV infections, compared to reverse line blot analysis [ 7].
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