Test results shows that proposed relaying schemes can provide primary as well as back-up protection to the forward and reverse line sections respectively.
Diagnosis was confirmed by polymerase chain reaction and reverse line blot and dogs infected with Babesia canis vogeli or Ehrlichia canis were excluded.
The advantage of this scheme is to provide a major protection up to 99.5% of total line length using single end data and furthermore backup protection to the forward and reverse line sections.
The methods used were polymerase chain reaction (PCR) and reverse line blot (RLB) hybridization.
Thus, reverse line probe assays are used as a first step analysis tool.
Initially, we performed HPV detection and typing on all cases using nested PCR and reverse line hybridisation.
Moojen et al. (2007) published a study on the optimization and validation of a combined broad-range 16S rRNA PCR and reverse line blot hybridization protocol.
HPV testing was performed using biotinylated L1 consensus primers and reverse line blot in cervical samples from women and in genital samples from men.
For example, enzyme immunoassay, which was used in our study, is more likely to detect the excess of multiple HPV infections, compared to reverse line blot analysis [ 7].
For confirmation of norovirus and strain genotyping, samples were further assayed by open reading frame 1 reverse transcription PCR (RT-PCR) by using biotin-labeled JV12 ad JV13 primers and reverse line blot hybridization (RLBH) (12 ).
Data were entered by using the Bionumerics (BN) package (Applied Maths, Ghent, Belgium), which allows storage, comparative analysis, and clustering of combined epidemiologic and biological experimental data (e.g., sequences, reverse line blot results, enzyme immunoassay data).
