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Regular and reversed light:dark cycle rats were anaesthetized with isoflurane (1 3% in O2, inhaled).
Matings were conducted during the dark phase of a 10 14 hour reversed light:dark cycle, under a red light.
The animals were housed in suspended wire cages on a 12 hr:12 hr reversed light cycle with lights off at 12 00 hours.
Nocturnal animals in zoos are generally housed on reversed light cycles and are illuminated by blue or red artificial light so that daytime visitors can observe their active behaviors.
Light exposure in the reversed light:dark cycle animals was 10 mins until the anesthesia took effect.
Rats were divided into two groups and entrained to either a regular or reversed light:dark cycle (see Methods).
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In one room, lights were on from 09 00 to 21 00 and in the other room, lights were on from 21 00 to 09 00 (DL12 12; reversed lighting regimen).
Timed-pregnant mice were held in a 12∶12 h reversed light-dark cycle (lights on at 19 00), and temperature and humidity-controlled (21±1°C, 55±5%) animal vivarium.
All animals in the two-bottle-choice experiments were maintained on a 12 hour reversed light-dark cycle (lights off at 10 am) and rats in the operant self-administration and locomotor experiments were maintained on a regular 12 hour light-dark cycle (lights on at 7 am).
The light dark cycle was reversed (lights on 19 00 7:00).
Reversed lighting conditions were used for sexual behavior studies.
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