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Endocrine Subscale items are negatively framed; thus, scores were reversed for analysis so that high scores equate to a good QoL.
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Note: item 10 score was reversed for factor analysis.
To ease interpretation, the rating scale of the IVI was reversed for Rasch analysis so that patients with a high level of VRQoL were given high scores.
Because the FACT-G includes some items that are worded positively, the responses to all negative items were reversed for all analysis.
The SF-36 summary and dimension scores were reversed for this analysis, i.e. the lower the score, the better the quality of life.
The categories (1 = never, 2 = some of the time, 3 = most of the time and 4 = all of the time) were reversed for Rasch analysis so that higher values on the logit scale corresponded to higher levels of well-being.
Where appropriate, responses to questions were reversed for data-analysis purposes so that all responses could be combined to produce a unidirectional overall assessment.
Two items were negatively worded and reversed for the statistical analysis.
Two items were negatively worded and were reversed for the statistical analysis.
*Statements were reversed for scoring Data analysis was undertaken using SPSS (version 12.0) and STATA (version 9.0).
In general, longer incubation causes permanent cross-linking which may be difficult to reverse for successful PCR analysis (20).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com