Sentence examples for reverse transcript from inspiring English sources

A total reaction volume of 20 μl was used for reverse transcription; 2 μl of reverse transcript buffer, 0.8 μl (100 mM) dNTP, 2 μl random priming oligonucleotides, 1 μl of MultiScribe™ Reverse Transcriptase (50 U/μl) (Applied Biosystems), 4.2 μl of nucleic acid-free water, and 10 μl (1000 ng) of total RNA.

Total peripheral leukocyte RNA was extracted from 50 AS patients and 161 healthy controls by the TRIzol reagent method (Invitrogen, Carlsbad, CA, USA), then underwent reverse transcription by use of a reverse transcript kit (Takara, Otsu, Shiga, Japan).

The late reverse transcript primer set spans the reverse transcription primer binding site and amplifies all cDNA forms including complete linear cDNA, 1LTR and 2LTR circles, and integrated provirus [28].

In all, 0.5 1  μg of RNA was reverse transcript using the kit ImProm II Reverse Transcription System (Promega, Charbonnieres, France) according to the manufacturer's instructions.

After reverse transcript reaction from 1 μg of WAT and BAT mRNA, quantitative realtime reverse transcription polymerase chain reaction (RT-qPCR) was performed in a CFX96TM Real-Time PCR Detection System (Bio-Rad, Hercules, CA) and the FAM dye label format for the TaqMan® Gene Expression Assays (Applied Biosystems).

To investigate the transcription of lysozyme genes in eggs and accessory glands, we performed reverse transcript (RT -PCR analysis using mRT -PCRlanalysism the salivary glands and legs of workers, the accessory glands of qusing, imRNAure eggs collected from queen ovarisolatedoviposited eggs collected from a natheal colony.

Over a period of 11 weeks, cellular uptake of the DNA vaccine was examined by PCR, transcription of the insert by reverse transcript-PCR (RT-PCR) and mRNA translation by immunofluorescence staining of muscle biopsies.

At 48 h after transfection, treated cells were harvested for reverse transcript-polymerase chain reaction (RT-PCR).

Reverse transcript-polymerase chain reaction (RT-PCR) analysis was used, wherever possible, to investigate frameshift, nonsense and splicing mutations to assess their effect on GALC mRNA processing.

Real time quantitative reverse transcript-polymerase chain reaction (qRT-PCR) with SYBER Green, using gene-specific PCR primers, was performed to verify the microarray data.

Presence of HCV RNA viremia was confirmed by nested reverse transcript-polymerase chain reaction (RT PCR), using Roche Amplicor, the detection limit being 100 copies per ml.