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Forward and reverse sequencing reactions were performed on an ABI 3730 automated sequencer using ABI Prism BigDye Terminator version 3.1 cycle sequencing (Applied Biosystems, Foster City, CA, USA).
The 5′ termini of the forward and reverse primer contain an extra 22nt sequence (underlined sequence) respectively homologous to 6His tag region and BGH reverse sequencing primer biding region in pC3.3.
Both forward and reverse sequencing reactions were performed, thus providing two sequencing reads for each clone.
Forward and reverse sequencing reactions were conducted for all PCR products.
High quality sequence traces were obtained for all 7 products from both the forward and reverse sequencing reactions and from the nested reactions.
(Malaysia) for forward and reverse sequencing using M13 primers.
Several Rad51 variants were identified and confirmed by reverse sequencing.
All forward sequencing results were confirmed by reverse sequencing.
This subject presented with a heterozygous genotype, confirmed by repeated forward and reverse sequencing.
For each sequenced accession, forward and reverse sequencing reactions were performed for increased coverage.
The SNPs positions and individual genotypes were further confirmed by re-amplification and reverse sequencing.
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