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The spectrum of mutations identified through forward and reverse screens using EMS, although similar at the level of DNA sequence is much different when the effects of the mutations are compared.
Individual M2 plants harboring putative hemizygous deletions or duplications within the ∼1-Mb syntenic intervals on chromosomes 8 and 15 were identified based on reverse screens (explained above) and aCGH analysis.
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It also conducted reverse screening of every passenger who got off a United flight that left San Francisco in that period, checking shoes and baggage at the various destinations.
Reverse screen was initiated among 192 M4 genomic DNA pools.
In fast neutron treated population, it is not uncommon to identify multiple mutated genes from reverse screen.
Gene-specific primers were designed for polymorphic coding sequences of the seven candidate genes for reverse screening.
Reverse screening based on the polymorphic coding sequence of seven Fe homeostatic genes detected by denaturing high performance liquid chromatography (dHPLC) revealed MuFRO1, a mutant for OsFRO1 (LOsFRO14g36720).
Forward (screening for phenotype) and reverse (screening for activity against a selected protein) chemical genetics, by combining medicinal chemistry, biological screening and combinatorial synthesis techniques, has enabled us to address previously intractable problems.
For Fe toxicity screening, 4,500 lines were randomly chosen for forward screening while 500 pooled DNA libraries (representing 24 M1 plants per pool) from the M4 generation were used for reverse screening (Figure 1). Figure 1 Schematic view of mutant discovery for rice mutant tolerance to Fe toxicity by reverse and forward genetic screens of a large FN-treated population.
The Herbochip® technology reported here was basically a reverse screening method.
The reverse screen subpopulation recovered the highest average number of duplication events.
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