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20 cycles of PCR amplification were performed in the presence of 5 mM MgCl2, 100 μM dNTPs, 1 μM each forward primer (5′ ATCGTAGGCACCTGAAA) and reverse primer (same as RT primer), 1X Taq buffer and 2.5 Units Taq polymerase (UBI).
If industry consolidation trends reverse, same.
PCR products that produced clear single bands were purified using ExoSAP-IT for PCR Product Clean-Up (Affymetrix) and then sequenced directly in two reactions with forward and reverse primers (the same as the amplification primers).
Mr. Coffin said the decision was "consistent with the trend of the Rehnquist court seeing discrimination and reverse discrimination as the same thing".
A positive and negative sign of SC correspond, respectively, to a response in the same or reverse direction as the variation in the parameter or input.
Since a ban on abortions after 20 weeks would not be consistent with post-Roe precedent, would upholding such a law effectively be the same as reversing Roe?
But it is important to remember that de Blasio agreeing to court monitor is not the same as "reversing" the program — and that in 2013 a federal judge even said the program had decreased and the crime rate has continued to fall.
Cycle sequencing in both directions was achieved using the same forward and reverse primers as for the RT-PCRs.
Sequencing was done in both directions using the same forward and reverse primers as those used in the PCR.
The purified mtDNA and nuclear DNA fragments were subject to direct sequencing in the forward and reverse directions using the same forward and reverse primers as in the PCR and the BigDye Terminator 3.1 Cycle Sequencing Ready Reaction Kit (Applied Biosystems).
The sequences of the RNU6B primers used were forward, 5′-CTCGCTTCGGCAGCACA-3′ reverse, 5′-AACGCTTCACGAATTTGCGT-3′ the sequence-specific reverse transcription primer of RNU6B was the same as its reverse PCR primer.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com