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This is due to the excessive penalty paid by a reverse polymerase at this high temperature.
In the case of a reverse polymerase, when the same sort of comparison indicated a spontaneous hydrolysis, the addition loop was halted until the next simulation time step.
At these higher temperatures, the effect on growth appeared to be greater on organisms containing the reverse polymerase than on those containing the forward polymerase.
This leaves a question of whether allowing mutations, which affect the generation to generation inheritance of polymerase rate, allow for a more rapid resolution of the more dominant strategy, or whether the addition of mutation to a reverse polymerase strategy causes this to be a loosing strategy when it otherwise might not be.
cDNA was prepared using SuperScript II reverse polymerase (Invitrogen) using random hexamers according to manufacturer's protocol.
All study patients were confirmed H1N1 positive by real-time reverse polymerase chain reaction (PCR) test.
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Because the probability of a hydrolysis event also depends on time, the Boltzmann distribution was scaled inversely with polymerase rate for the reverse polymerases.
reverse transcript polymerase chain reaction.
reverse transcription polymerase chain reaction.
quantitative reverse transcription polymerase chain reaction.
Semi-quantitative reverse transcriptase polymerase chain reaction.
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