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We identified nine chromosomal segments flanked by syntenic sequences but were in reverse orientations between the H99 and JEC21 genomes.
NKX2-5 cDNA was cloned into SalI/BglII site of pAdTrack-CMV (Stratagene Inc). in forward and reverse orientations.
All the sequences were used in the correct and reverse orientations, forming a database with 13,760 entries.
Since ID elements tend to integrate in both forward and reverse orientations, we also examined the insertion of an ID1 element in the reverse orientation.
Sanger-verified true positive variants showed complete agreement in both forward and reverse orientations and these variants were located within high-quality regions (Phred score ≥20).
As the name implies, a simple inversion is defined as one stretch of a chromosome, flanked by syntenic chromosomal regions but in reverse orientations in the H99 and JEC21 genomes.
Similar(29)
It is flanked by perfect direct repeats (one orientated in reverse orientation) and is integrated between two asn tRNAs, of which one in the reverse orientation.
However, this region is flanked by perfect direct repeats (one orientated in reverse orientation), and it is integrated between two asn tRNAs, of which one is in the reverse orientation.
Interestingly, this GGD /E EF protein contains a EAL site in the reverse orientation.
It can be seen that the C-V curve moved slightly towards a reverse orientation after SiN passivation.
The reverse orientation produced superior granule size distribution with improved liquid distribution attributes compared to the forward orientation.
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