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It is flanked by perfect direct repeats (one orientated in reverse orientation) and is integrated between two asn tRNAs, of which one in the reverse orientation.
However, this region is flanked by perfect direct repeats (one orientated in reverse orientation), and it is integrated between two asn tRNAs, of which one is in the reverse orientation.
The FLEX-mDAT-pACP plasmid consists of a FLEX cassette, into which the mouse dopamine transporter (mDAT) cDNA was inserted in the reverse orientation.
Interestingly, this GGD /E EF protein contains a EAL site in the reverse orientation.
It can be seen that the C-V curve moved slightly towards a reverse orientation after SiN passivation.
The Ter sequence has been presented in reverse orientation [11].
It codes for the essential D4R gene in reverse orientation.
As controls we transfected empty vectors or vectors with the 5'UTRs in reverse orientation.
In KI-MULEs, the ULP protease domain is found in the reverse orientation with respect to the mudrA gene.
Again, as a control, dfoxO IRES translation was inactive with the 5'UTR inserted in reverse orientation (Fig. 3B).
Plasmid pHPI-8234 walsolso constructed, which contains the XhoI fragment of HCV1a (H strain) in the reverse orientation.
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