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Average reverse flux was accurately measured by two different methods leading to the same results.
Moreover, the models do not accurately predict the average reverse flux when the suspension interacted with the membrane.
We find the flow within the centre of the gyre is surprisingly quiescent, for example in the vicinity of the South Atlantic reverse flux patches.
The average reverse flux could even be higher than the forward flux, which could hamper the economical achievement of the technique.
The experimental set-up of this work allowed obtaining the forward flux response to periodical backpulsing and measuring the average reverse flux.
The field morphology is similar to previously published maps for the satellite era, which includes the large reverse flux patch in the southern hemisphere and the sinuous magnetic equator line.
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For some reversible reactions, we measured both forward and reverse fluxes and calculated net fluxes towards product synthesis.
For reversible carbon exchange reactions both forward and reverse fluxes were determined and used to calculate net fluxes toward product synthesis.
The retention times and mass-to-charge (m/z) ion clusters of selected ions of cells and culture media metabolites were determined using mass isotopomer distribution analysis (MIDA) expressed as net fluxes by subtracting reverse fluxes from forward tracer incorporation patterns via reversible metabolic steps.
The retention times and mass-to-charge (m/z) ion clusters of selected ions of bacterial and culture media metabolites were determined using mass isotopomer analysis (MIDA) [ 27, 28], and expressed as net fluxes by subtracting reverse fluxes from forward tracer incorporation patterns via reversible metabolic steps [ 29].
Here we introduce a theorem that relates forward and reverse fluxes and free energy for any chemical process operating in a steady state.
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