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Upon reaching the diagnostic chip, electronic hybridization was performed for a competitive reverse dot blot assay.
Combined experiments using reverse transcriptase polymerase chain reaction (RT-PCR), colony hybridization and reverse dot blot yielded three distinct probes.
The results of evaluating 139 blinded samples by our system match perfectly with those obtained by the conventional reverse dot blot (RDB) method.
The new multiplex asymmetric polymerase chain reaction and asymmetric hyper-branched rolling circle amplification coupled with reverse dot blot (RDB) systems were developed to detect GMCs.
DNA-chip technology, Papillocheck® HPV-screening (Greiner) and reverse dot blot, Linear Array (LA) (Roche) are tools to assess the distribution of HPV genotypes.
The detection of these mutations is currently performed by the reverse dot blot (RDB) hybridization technique, which could detect only known mutations.
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In the future these kits that use the classical reverse dot-blot approach will be replaced by micro-arrays, DNA chips and Labs on a chip.
Several techniques such as allele specific oligonucleotide (ASO) dot-blot, reverse dot-blot, amplification refractory mutation (ARMS), and an oligo-ligation assay, are available to detect the most common mutations.
The exact genotypes of these samples were determined previously by reverse dot-blot analysis.
Extracts were analyzed further by reverse dot-blot hybridization of the M. tuberculosis complex-specific Direct Repeat (DR) region, a procedure known as spoligotyping [26].
Finally, the established MCPC assay was blindly tested with 239 clinical samples that have been previously analyzed by the reverse dot-blot method, 13 different genotypes (Figure 4) representing 12 patients, 98 carriers, and 129 normal samples (Table S2) were accurately identified.
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