Reverse transcriptase PCR with the same amount of total RNA from each cell line taken for reverse transcription confirmed that all lines express PKC α mRNA.
Yes, the zodiac was laid out in reverse, he confirmed shortly after my tour.
The increase of the reverse current confirmed the photogeneration of free carriers in the structures under study.
We also performed the experiment in reverse and confirmed that immunoprecipitated HA-Grb2 pulled down FLAG-Dvl2 (data not shown).
A separate control containing taq polymerase and all other reagents except the reverse transcriptase confirmed that the band was not genomic DNA (not shown).
As shown in Figure 5, the reverse assay confirmed the interaction, finding PLP in extracts immunoprecipitated with anti-GFP polyclonal antibody.
Reverse BLAST confirmed assignment as DNAj-family ORFs, but due to the size and diversity of the DNAj families, orthologous relationships could not be unequivocally assigned as cytoplasmic, mitochondrial and ER members of the Hsp/DNAj family were returned.
PCR without reverse transcriptase confirmed the absence of DNA.
The fact that no amplification products were obtained in negative control reactions lacking reverse transcriptase confirmed that template RNA was free from contaminating genomic DNA.
Bronchoalveolar lavage viral culture was positive for influenza A, and real-time reverse transcription PCR confirmed pandemic (H1N1) 2009 virus infection.
