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[99mTc]Tilmanocept detected 364 nodes, for 63.7 % reverse concordance (232 of 364 nodes).
Reverse concordance was defined as the percentage of radioactive lymph nodes that were also blue.
Reverse concordance, the proportion of radioactive nodes detected by blue dye, was also calculated.
Another secondary end point, reverse concordance, evaluated the proportion of all nodes detected by [99mTc]tilmanocept that were also blue.
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Assembly accuracy was confirmed by forward and reverse read-pair concordance of individual plasmid subclones.
The program Arachne [14] was used to assemble the genome and no assembly errors were observed based on the concordance of forward and reverse read-pairs.
Expression calls from Affymetrix MAS 5.0 software were normalized before analysis, resulting in values that showed good concordance with data from SybrGreen reverse transcription-qPCR studies ([ 62]; Additional file 2).
Cycleave PCR utilises chimeric DNA-RNA-DNA probes labelled with a fluorescent dye and quencher, and the accuracy of cycleave PCR in detecting KRAS/BRAF mutations has been confirmed by assessment of the concordance between cycleave PCR and reverse transcriptase PCR-coupled direct sequencing (Yatabe et al, 2006; Yokota et al, 2010).
We then examined KLK6 mRNA expression using reverse transcription polymerase chain reaction and established its good concordance with KLK6 protein expression.
The intent of this study was to use HPV as a model system to assess the feasibility of electrochemical detection with the eSensor chip of multiple pathogens, not to achieve perfect concordance of HPV detection with the reverse line blot.
Reversing these percentages to ratios reveals a reasonable concordance (0.0204 for the K and 0.0213 for the Trp, changes).
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